Non-motor symptoms in a rat model of neurotoxicity induced by 6-OHDA: effect of intracisternal RAd IGF1 in anxiety – like behavior.

CHAMPARINI L; HERRERA M.; COMETTO M; NARANJO VITERI A,; CRESPO R; CALFA G; HEREÑU C

Granada

Congreso; Congreso IBRO 2023; 2023

Parkinson's disease is a neurodegenerative disorder characterized by the progressive loss of dopaminergicneurons, resulting in a range of motor and non-motor symptoms. Although motor deficits are the main criteria fordiagnosis, there is growing evidence that non-motor symptoms may also play an important role in the diseaseprogression. In this study, we aimed to investigate the onset and progression of cognitive and anxiety-like behaviordeficits in a rat model of neurotoxicity induced by 6-OHDA infusion into the dorsolateral striatum. We assessed thebehavioral changes in male Wistar rats after 1, 2 and 3 weeks post-lesion using the Y-maze, elevated plus maze,light-dark box test and locomotor activity test. Our results showed anxiety-like behavior at 2 and 3 weekspost-lesion, before the onset of motor impairment. We also observed working memory impairment in 6-OHDA ratsafter 3 weeks of neurodegeneration, without motor alterations. Immunohistochemical analysis revealed a decreasein tyrosine hydroxylase immunoreactivity in the substantia nigra, ventral tegmental area, and striatum, indicatingpartial lesion of the nigrostriatal dopaminergic system. Furthermore, we found an increase in mRNA levels ofproinflammatory cytokines IL-1