Effect of geraniol on rat cardiomyocytes and its potential use as a cardioprotective natural compound

CRESPO R.; WEI K; RODENAK-KLADNIEW B; MERCOLA M; RUIZ-LOZANO P; HURTADO C

LIFE SCIENCES

PERGAMON-ELSEVIER SCIENCE LTD

Lugar: Amsterdam; Año: 2017 vol. 172 p. 8 - 12

0024-3205

Aims: Reactive oxygen species (ROS) are generated in the ischaemic myocardium especially during early reperfusionand affect myocardial function and viability. Monoterpenes have been proposed to play beneficial roles indiverse physiological systems; however, the mechanisms of action remain largely unknown. This study aims toassess the effect of monoterpene geraniol (GOH) on neonatal rat ventricular cardiomyocytes (NRVCs) subjectedto oxidative stress.Main methods: We used an in vitro model of hypoxia-reoxygenation. Cardioprotective (AMPK) and cardiotoxic(ERK1/2, ROS) signaling indicators were measured. Assays were performed by fluorogenic probes, MTT assaysand Western-blots.Key findings: We determined that the addition of GOH (5?200 μM) to cultured normoxic and hypoxic-NRVCs diminishedthe endogenous production of ROS in stressed cardiomyocytes. We observed that GOH treatment increasedpAMPK levels and decreased pERK1/2 levels in cultured NRVCs.Significance: This report suggests that GOH is a candidate cardioprotective natural compound that operates byblunting the oxidative stress signaling that is normally induced by hypoxia-reoxygenation.