CONSEQUENCES OF MATERNAL FRUCTOSE INTAKE ON BROWNING POTENTIAL OF RETROPERITONEAL ADIPOSE TISSUE FROM ADULT OFFSPRING

ALZAMENDI A; SPINEDI E; GIOVAMBATTISTA A

Congreso; Reunión Conjunta de Sociedades de Biociencias; 2017

Beige adipocytes are highly adapted to convert chemical energy into heat through the action of uncoupling protein-1 (UCP1). Cold exposure or β3 adrenergic agonist treatment stimulates generation of these cells in white adipose tissue (WAT). Our aim was to assess whether maternal fructose intake during pregnancy, affects browning capability of retroperitoneal adipose tissue (RPAT) from adult male offspring. On pregnancy day 1, dams were provided with either tap water alone (CTR, control) or containing fructose (10%w/v; FRD) and fed ad libitum with chow up to delivery. Lactating dams and their pups (between 21 and 60 days) received water and chow ad libitum. C and F indicate pups born to CTR and FRD dams. On experimental day (age 60 days) RPAT was dissected and stromal vascular fraction (SVF) cells were isolated. mRNA expression levels of beige and white adipogenic markers were assessed in RPAT SVF cells and pads. SVF cells were cultured and differentiation parameters were quantified by qPCR. Previously we found that pre-natal nutritional intervention decreased the adipogenic potential of adult RPAT precursor cells, favoring hypertrophic RPAT expansion and a distorted adipokine secretion pattern. Now, we found that F RPAT expresses lower levels (p