EFFECT OF ESTRADIOL ON THE ADIPOGENIC PROCESS IN RETROPERITONEAL AND INGUINAL ADIPOSE TISSUE OF FEMALE RATS

MIGUEL I; ALZAMENDI A; MARTIN F; REY A; SPINEDI E; GIOVAMBATTISTA A

Congreso; Reunión Conjunta de Sociedades de Biociencias; 2017

The mechanism by which estradiol (E2) regulates adipose tissue (AT) development are largely unknown. Previously, we have shown an in vitro E2-dependent decreased adipogenic potential in retroperitoneal adipose tissue (RPAT) adipocyte precursor cells. In this study, our aim was to assess the role of E2 deficiency on RPAT and inguinal AT (IAT) pads in female rats. S-D rats were divided into three groups: non-ovariectomized (Sham operated, CTR), ovariectomized (OVX) and ovariectomized pair-fed (OVX PF), latter ones castrated prepubertally. CTR and OVX food consumption was ad libitum, while for OVX PF was the same to CTR, due to a higher food consumption of OVX rats. Food intake and body weights were recorded daily from surgery (27-day-old) to experimental day (60-day-old). At this time, trunk blood was collected to measure plasma metabolites. RPAT and IAT pads were dissected, weighed and histological analysis were performed. Moreover, stromal vascular fraction (SVF) from both tissues was isolated to test mRNA levels of PPARγ (adipogenic marker) by qPCR. OVX PF animals show a decrease in RPAT mass and %RPAT (OVX PF P