Maternal fructose rich diet intake throughout gestation inhibits the adipogenic potential of white adipose tissue precursor cells from the male offspring

ALZAMENDI A; ZUBIRÍA G; PORTALES A; SPINEDI E; GIOVAMBATTISTA A

Mar del Plata

Congreso; LXI Reunión de la Sociedad Argentina de Investigación Clínica; 2016

Sociedad Argentina de Investigación Clínica

Previous studies from our group indicate that fructose rich diet (FRD) consumption during gestation induces a decrease in adipose precursor cells (APCs) from retroperitoneal adipose tissue (RPAT) thus reducing local adipocyte number and mass in the adult male progeny. These changes favor adipocyte hypertrophy and a distorted adipokine secretion pattern. We now evaluated the impact of FRD intake by the gestating dams on the adipogenic capacity of stromal vascular fraction (SVF) cells from RPAT, of their adult male progeny. On pregnancy day 1, dams were provided with either tap water alone (control) or containing fructose (10% w/v in drinking water; FRD) and fed ad libitum with chow up to delivery. Lactating dams and their pups (between 21 and 60 of age) received water and chow ad libitum. C and F animals will come from control and FRD dams, respectively. On experimental day (age 60 days) RPAT pads were dissected and SVF cells were isolated. The mRNA expression levels of adipogenic potential markers were assessed by qPCR in RPAT SVF cells and pads. PPARγ expression was quantified in differentiating cells and markers of terminal differentiation were also evaluated. Our data indicate that local F SVF cells express lower and higher levels (p