Insecticidal activity of essential oils from native medicinal plants of Central Argentina against the house fly Musca domestica (L.)

SARA M. PALACIOS, ; ALBERTO BERTONI; YANINA ROSSI ; ROCIO SANTANDER; ALEJANDRO URZÚA

PARASITOLOGY RESEARCH

SPRINGER

Año: 2009 vol. 106 p. 207 - 212

0932-0113

The insecticidal activity of nine essential oils(EOs) against the house fly (Musca domestica) was evaluated by placing flies in a screw-cap glass jar holding a piece of EO-treated cotton yarn. The dose necessary to kill 50% of flies (LC50) in 30 min was determined at 26± 1°C. The EOs showed LC50 values ranging from 0.5 to 46.9 mg/dm3. The EO from Minthostachys verticillata was the most potent insecticide (LC50=0.5 mg/dm3) followed by EOs from Hedeoma multiflora (LC50=1.3 mg/dm3) and Artemisia annua (LC50=6.5 mg/dm3). The compositions of the nine EOs, obtained by hydrodistillation of medicinal herbs, were analyzed by gas chromatography/mass spectroscopy. These analyses showed that (4R)(+)-pulegone (69.70%), menthone (12.17%), and limonene (2.75%) were the principal components of M. verticillata EO. (4R)(+)-pulegone was also the main constituent (52.80%) of H. multiflora, while artemisia ketone (22.36%) and 1,8-cineole(16.67%) were the major constituents of A. annua EO. The terpene (4R)(+)-pulegone showed a lower toxicity (LC50= 1.7 mg/dm3) than M. verticillata or H. multiflora EOs. Dimethyl 2,2-dichlorovinyl phosphate, selected as a positivecontrol, showed an LC50 of 0.5 mg/dm3. EOs from M. verticillata and H. multiflora show promise as natural insecticides against houseflies.