Evaluation of cell damage produced by Fe ions released as degradation products of biomaterials. Influence of pH changes.

FAGALI NS; GRILLO CA; PUNTARULO S; FERNÁNDEZ LORENZO MA

Maratea

Congreso; 6th Symposium on Biodegradable Metals; 2014

Pure Fe and some of its alloys have been proposed for the manufacture of temporary stents because they are susceptible to a fast corrosion in biological media. However, the release of Fe ions during dissolution may cause side effects like oxidative stress and inflammation in the tissues in contact with the implant. Despite its importance, the mechanisms by which these deleterious effects are produced have not been sufficiently described in the literature. In this work, the effect of Fe2+ and Fe3+ ions and pH changes on cultures of CHO-K1 cells was evaluated. CHO-K1 cells were exposed to various concentrations of Fe2+ and Fe3+ salts (1-5 mM range) and mitochondrial activity (MTT technique), oxidative damage to lipids (TBARS determination) and intracellular Fe (bathophenanthroline technique) were determined. To evaluate Fe effect the Fe+2 solutions were prepared in acid culture medium (CM, pH