CONICET | Buscador de Institutos y Recursos Humanos

The interactions that could be built between the biomaterials and tissue microenvironments are very complex, especially in case of degradable metals that generate a broad variety of degradation products. The interfacial problems are particularly relevant for Fe-based materials that have been proposed for the development of biodegradable implants. The cell metabolism could be affected by the accumulation of insoluble Fe-containing degradation products that has been observed in vitro and in vivo as a coarse granular brownish material around the implant. However, the relative importance of each Fe-species (soluble and insoluble) on the cellular behavior of the surrounding cells, particularly on the generation of reactive species (RS), is not completely elucidated. The aim of this study is to evaluate the processes occurring at the Fe biomaterial/cells interfacial region, and to discriminate the effects of soluble and insoluble corrosion products released by the bulk metal (Fe- microparticles (Fe0p) or Fe0ring) on the adjacent cells, mainly in relation to RS generation. With this purpose Fe0p and Fe0ring were incubated with fibroblast cells (BALB/c3T3 line) for 24 and 48 h periods. Then different techniques were used, such as the dichlorofluorescein diacetate assay (DCFH2-DA) for detection of RS, acridine orange dye for cell viability, total protein content determinations, Prussian Blue staining and TEM observations. To individualize the effects of soluble and insoluble species, independent experiments with Fe3+-salts were performed. Overall data indicate that RS generation by cells exposed to the degradation products of Fe-based biomaterials is more dependent on the presence of insoluble products than on soluble Fe species.

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