Video Tracking Analysis System for Forelimb Akinesia in the rat Parkinson Model

BONACCORSO P.; LEDESMA MJ.; NIETO GRIMALT FE.; CABRERA R.

Bucaramanga

Congreso; VII Congreso Latinoamericano de Ingenieria Biomédica; 2016

Sociedad Latinoamericana de Ingenierí biomédica

This study analyzes forelimb akinesia with steppingtest in adult male rats with nigrostriatal cell loss and striataldopa-mine depletions, similar to levels reported in the clinicalsetting; and determines if subjects who received a pharmacologicalinduction with apomorphine exhibit more robust parkinsonianeffects. We present the development of software toevaluate this test and we correlate results with those obtainedfrom manual recording. Adult rats received infusions of 6-hydroxydopamine (6-OHDA) in the left corpus striatum. 2 monthsafter lesion they were tested for forelimb akinesia using steppingtest, before and after an apomorphine induction. Video trackinganalysis system analysis consists of two stages. The first, whereanimals performance is recorded and their trajectories are acquiredand processed using open-source software. In the secondone, post-processing methods are conducted to measure steppingparameters, such as adjusting steps, stepping time and initiationtime. Based on these measurements, the program has theability to make statistical analysis and present data in differentformats, including matrixes and graphs. There are many advantagesto using such a computerized system. Some of them are thepossibilities to decrease inter/Intra operator errors due to directobservation and handling, to expand the number of parametersassessed, to early and acutely detect those para-meters and toincrease speed and quality of data. Our results provide some interestingcues regarding what could be a useful area of researchin order to use bioinformatics to assess ethological behavior inanimal models in different stages of Parkinson disease. What ismore, we could establish a relationship between manual andautomated recording.