Positive effects of orchiectomy on swimming behavior in hemiparkinsonian animals treated with the neuroactive steroids estrogen and progesterone

GONZALEZ NATALIA; CABRERA RICARDO

Virtual

Congreso; Reunión conjunta SAIC, SAI, AAFE, NANOMED.AR; 2021

SAIC, SAI, AAFE, NANOMED.AR

Parkinson’s disease is the second most frequent neurodegenerative pathology after Alzheimer’s disease, with a predominance in men over women. Unilateral neurotoxic injury with 6-hydroxydopamine (6-OHDA) induces selective dopaminergic neurodegeneration andhemiparkinsonism in rats with contralateral predominant motor impairment.The aim was to evaluate whether the decrease in testosterone concentration by orchiectomy (ORX) would be a positive factor on the neuroprotective effect induced by estrogen (E) and progestrone(P) administration on the motor and depressive-like behavior alterations observed in the injured animals. Male Sprague-Dawley rats (280-320 g) aged 60 days ORX and non-ORX were used.At 10 days post ORX, neurodegeneration was induced by microinjection of 6-OHDA into the left striatum. The experimental groups were 1- non-ORX: control (C), E and P-treated control (CEP), hemiparkinsonian (HP), E and P-treated hemiparkinsonian (HPEP) and 2-ORX: control (CO), E and P-treated control (COEP), hemiparkinsonian (HPO), E and P-treated hemiparkinsonian (HPOEP). Signsof motor impairment and hopelessness were assessed by a forced swim test (PNF). Data were expressed as mean+SEM and analyzed by ANOVA 2 and Student Newman-Keuls.ORX induced a significant increase in swimming time in HP animals with respect to group C (p