MOLECULAR GENETIC VARIABILITY DIVERSITY AND RELATEDNESS OF FASCIOLA HEPATICA ISOLATES FROM DIFFERENT HOSTS AND GEOGRAPHIC REGIONS USING THE RAPD-PCR TECHNIQUE

SCARCELLA SILVANA; MIRANDA - MIRANDA ESTEFAN; NEIRA GISELA; COSSÍO - BAYÚGAR RAQUEL; MERA Y SIERRA ROBERTO; SOLANA HUGO

Congreso; FLAP XXIV Congreso Latinoamericano de Parasitología; 2017

MOLECULAR GENETIC VARIABILITYDIVERSITY AND RELATEDNESS OF FASCIOLAHEPATICA ISOLATES FROM DIFFERENT HOSTS AND GEOGRAPHIC REGIONS USING THERAPD-PCR TECHNIQUEScarcella, Silvana 1; Miranda-Miranda, Estefan 2;Neira, Gisela 3; Cossio-Bayugar, Raquel 2; Mera y Sierra,Roberto 3; Solana, Hugo.11 Laboratorio de Biología  Celular y Molecular. Centro de InvestigaciónVeterinaria de Tandil (CIVETAN), CONICET, Facultad de Ciencias Veterinarias,UNCPBA, Tandil, Argentina2         Centro Nacional deInvestigación Disciplinaria en Parasitología Veterinaria, Instituto Nacional deInvestigaciones Forestales Agrícolas y Pecuarias. Jiutepec, México.3         Centro deInvestigación en Parasitología Regional, Universidad Juan Agustín Maza,Mendoza, Argentina.Fascioliasis is an important parasitic diseaseof domestic animals and humans worldwide, causing large economic losses to thelivestock industry and producing ever-increasing public health awareness as aneglected zoonotic parasitosis. Studies regarding the geneticconstitution as well as status of genetic variation of the parasite populationshave significant implications on the epidemiology and successful design ofcampaigns destined for the control of fasciolasis.The present communication focuses on molecular characterization of F.hepatica isolates usingrandom-amplified polymorphic DNA polymerase chain reaction (RAPDs- PCR) derivedfrom sheep, cattle and buffalo, collected between 2013 and 2016 in differentregions of Argentina. Adultflukes were collected fromdifferent hosts from abattoirs in diverse regions of Argentina. Flukes wereidentified morphologically as F. hepatica according to existing keys. Thegenomic DNA of each sample was isolated following the standardphenol?chloroform procedure. Duplicate PCR reactions on each individualtemplate DNA were performed to test the reproducibility of the individual DNAbands. DNA was resolved by agarose gel electrophoresis. The size of thefragments was determined by the Rf. Digital processing of amplicon bands was done using Labworks 4.0 gelimage analysis software. Genetic distances between genotypes were calculated onthe basis of Pearson?s coefficient algorithm for dendrogram construction. The dendrogram obtained contained two majorgroups at 50,047 and 50,613 which clearly diverged from the RAPDS markerswithin the genome of the parasites, but showed no correlation with respect tothe origin of the host, except for samples obtained from the same host specieswith a Genetic distance of 16 or less. Our results suggest that F.hepatica have different patterns of population genetic structure, which isconsistent with a parasite maintaining similar infectivity across several hostspecies with little or no importance of the infective-stage host origin,results also suggest that genetic variability depend more of the differentgeographical regions than of the definitive hosts