INVESTIGADORES
SCARCELLA Silvana Andrea
congresos y reuniones científicas
Título:
DIFFERENTIAL MOLECULAR CHARACTERIZATION OF FASCIOLA HEPATICA IN SEVERAL HOSTS OF CAJAMARCA, PERÚ. HIS IMPLICATION IN THE DEVELOPMENT OF ANTHELMINTIC RESISTANCE
Autor/es:
SCARCELLA, S.; FIEL, C.; SILVA DÍAZ, H.; ORTIZ OBLITAS P.; SOLANA, H.
Reunión:
Congreso; 24th International Conference of the World Association for the Advancement of Veterinary Parasitology; 2013
Resumen:
Fasciolosis caused by the trematode Fasciola hepatica is a parasitic disease
prevalent in ruminants raised in temperate regions of the world. This parasitism is
maintained in the field for their ability to infect different species such as cattle, sheep
or pig among others. It is economically
important with losses due to infection estimated as US$ 2000?3000 million
yearly. The infection affects up to the 75% of dairy cattle raised in the
northern region of Cajamarca, Peru. Furthermore, fasciolosis is an emergent zoonotic
disease in many countries, including this region of Peru, where has been
reported a prevalence of 24.4% in school children. Given the epidemiological
and economic importance of this disease, it is necessary to have information
relating to genetic variants in this trematode. Using the RAPD-PCR technique, inthis paper we evaluate the molecular characteristics of adult flukesfrom three different hosts, sheep, cattle and
pigs. We compared the
genetic profiles of F. hepaticafrom different hosts detected a differential expression of polymorphic fragments (RAPD) betweenthe species under study. The results obtained confirm
a high level of polymorphism in the genome of F. hepatica showing a manifest genetic variability between flukes from different hosts. It is valid postulate that this polymorphism
enhanced the adaptability in the host and at the environment. For
any selection pressure, whether natural or artificial (bad use
of fasciolicides and / or control and management measures), the existence of different genetically
distinct populations within the species, allow one or more populations of F. hepatica can survive and create resistance
or adaptability to this selective pressure