INVESTIGADORES
SCARCELLA Silvana Andrea
congresos y reuniones científicas
Título:
APROACH TO MOLECULAR CHARACTERIZATION OF FASCIOLA HEPATICA ISOLATES SUSCEPTIBLE AND RESISTANT TO TRICLABENDAZOLE BY TECHNIQUE RAPDS-PCR
Autor/es:
SCARCELLA, S.; CEBALLOS, L.; SANABRIA, R.; ORTIZ OBLITAS P.; SOLANA, H.
Lugar:
Perth
Reunión:
Congreso; 24th International Conference of the World Association for the Advancement of Veterinary Parasitology; 2013
Resumen:
Liver flukes Fasciola hepatica
are among the causes of foodborne diseases of parasitic etiology. These
parasites cause significant public health problems and substantial economic
losses to the livestock industry. Its control is mainly based on the use of
triclabendazole (TCBZ), a halogenated benzimidazole thiol derivative which
shows excellent efficacy against both juvenile (immature) and adult
stages. Anthelmintic resistance in
parasitic of livestock is a chronic problem in the world. The intensive use of
TCBZ has resulted in the development of resistant liver flukes. In nematodes the resistance to the
benzimidazoles is caused by genetic changes in genes encoding β-tubulins.
In the case of F. hepatica TCBZ
resistant were not detected these genetic changes for which the resistance
obtained is from other genetic or metabolic changes. The present study shows
the comparative molecular characterization of F. hepatica
susceptible and resistant to TCBZ using the Random Amplified Polymorphic
ADN Fragments (RAPDs-PCR) technique. Were
evaluated ten different primers and four isolates of F. hepatica Cullompton
and CEDIVE (susceptibles) and Sligo and Cajamarca (resistant to TCBZ). The
results provides evidence of genetic variations of F. hepatica between
the different isolates, reinforcing previous studies where is proposed that the
resistance is due to a combination of factors, which vary among different
isolates.
Further work on this relevant area is required.
Acknowledgements:
TCBZ-susceptible isolate (Cullompton) and the
TCBZ- resistant (Sligo) were kindly provided by Professor I. Fairweather,
School of Biology and Biochemistry, The Queens University of Belfast, Northern
Ireland, UK.