MORFOLOGIA COMPARATIVA DEL APARATO REPRODUCTOR FEMENINO DE Fasciola hepatica SENSIBLE Y RESISTENTE A TRICLABENDAZOLE

SCARCELLA S., FELIPE A., ALZOLA R., REEVERG M., Y SOLANA H.

Huerta Grande, Cordoba

Congreso; 1° Reunion Conjunta de Sociedades de Biologia de la Republica Argentina, XVI Jornadas Cientificas de la Soc de Cordoba, IX Jornadas de la Soc Arg de Biologia y XXV Reunion Anual de la Soc de Biologia de Cuyo; 2007

Fascioliasis is a zoonosis of subtropical regions, with great incidence in  suburban population and great economic impact in cattle production. For its treatment triclabendazole  (TCBZ)  is used. Its  mechanism of action consists in  the despolimerization of F. hepatica microtubules with the consequent loss of function, loosening and death. Today, the undiscriminated use of these drugs  has generated the expression of the  anthelmintic resistance phenomena (loss of ovicide effect, among others). In the present work the structure of certain components of the female reproductive apparatus of two strains of F. hepatica was analyzed (TCBZ susceptible Fasciola, Cullompton strain, and TCBZ resistant Fasciola, Sligo strain). Conventional histology and immmunohistochemistry with anti á- tubulin was done for both strains . No morphological changes  were detected between both strains, so it is possible to especulate  that the resistance phenomena expression in the Sligo striain couldn’t be produced by modifications at genetic or metabolic level without detectable changes in morphological aspects. These results contribute to the understanding of the farmacological activity of this type of drugs contributing to the understanding of the mechanism of resistance to TCBZ by trematodes Fascioliasis is a zoonosis of subtropical regions, with great incidence in  suburban population and great economic impact in cattle production. For its treatment triclabendazole  (TCBZ)  is used. Its  mechanism of action consists in  the despolimerization of F. hepatica microtubules with the consequent loss of function, loosening and death. Today, the undiscriminated use of these drugs  has generated the expression of the  anthelmintic resistance phenomena (loss of ovicide effect, among others). In the present work the structure of certain components of the female reproductive apparatus of two strains of F. hepatica was analyzed (TCBZ susceptible Fasciola, Cullompton strain, and TCBZ resistant Fasciola, Sligo strain). Conventional histology and immmunohistochemistry with anti á- tubulin was done for both strains . No morphological changes  were detected between both strains, so it is possible to especulate  that the resistance phenomena expression in the Sligo striain couldn’t be produced by modifications at genetic or metabolic level without detectable changes in morphological aspects. These results contribute to the understanding of the farmacological activity of this type of drugs contributing to the understanding of the mechanism of resistance to TCBZ by trematodes Fascioliasis is a zoonosis of subtropical regions, with great incidence in  suburban population and great economic impact in cattle production. For its treatment triclabendazole  (TCBZ)  is used. Its  mechanism of action consists in  the despolimerization of F. hepatica microtubules with the consequent loss of function, loosening and death. Today, the undiscriminated use of these drugs  has generated the expression of the  anthelmintic resistance phenomena (loss of ovicide effect, among others). In the present work the structure of certain components of the female reproductive apparatus of two strains of F. hepatica was analyzed (TCBZ susceptible Fasciola, Cullompton strain, and TCBZ resistant Fasciola, Sligo strain). Conventional histology and immmunohistochemistry with anti á- tubulin was done for both strains . No morphological changes  were detected between both strains, so it is possible to especulate  that the resistance phenomena expression in the Sligo striain couldn’t be produced by modifications at genetic or metabolic level without detectable changes in morphological aspects. These results contribute to the understanding of the farmacological activity of this type of drugs contributing to the understanding of the mechanism of resistance to TCBZ by trematodes Fascioliasis is a zoonosis of subtropical regions, with great incidence in  suburban population and great economic impact in cattle production. For its treatment triclabendazole  (TCBZ)  is used. Its  mechanism of action consists in  the despolimerization of F. hepatica microtubules with the consequent loss of function, loosening and death. Today, the undiscriminated use of these drugs  has generated the expression of the  anthelmintic resistance phenomena (loss of ovicide effect, among others). In the present work the structure of certain components of the female reproductive apparatus of two strains of F. hepatica was analyzed (TCBZ susceptible Fasciola, Cullompton strain, and TCBZ resistant Fasciola, Sligo strain). Conventional histology and immmunohistochemistry with anti á- tubulin was done for both strains . No morphological changes  were detected between both strains, so it is possible to especulate  that the resistance phenomena expression in the Sligo striain couldn’t be produced by modifications at genetic or metabolic level without detectable changes in morphological aspects. These results contribute to the understanding of the farmacological activity of this type of drugs contributing to the understanding of the mechanism of resistance to TCBZ by trematodes Fascioliasis is a zoonosis of subtropical regions, with great incidence in  suburban population and great economic impact in cattle production. For its treatment triclabendazole  (TCBZ)  is used. Its  mechanism of action consists in  the despolimerization of F. hepatica microtubules with the consequent loss of function, loosening and death. Today, the undiscriminated use of these drugs  has generated the expression of the  anthelmintic resistance phenomena (loss of ovicide effect, among others). In the present work the structure of certain components of the female reproductive apparatus of two strains of F. hepatica was analyzed (TCBZ susceptible Fasciola, Cullompton strain, and TCBZ resistant Fasciola, Sligo strain). Conventional histology and immmunohistochemistry with anti á- tubulin was done for both strains . No morphological changes  were detected between both strains, so it is possible to especulate  that the resistance phenomena expression in the Sligo striain couldn’t be produced by modifications at genetic or metabolic level without detectable changes in morphological aspects. These results contribute to the understanding of the farmacological activity of this type of drugs contributing to the understanding of the mechanism of resistance to TCBZ by trematodes