HALLAZGOS HISTOPATOLOGICOS DEL ÓRGANO REPRODUCTOR MASCULINO DE Fasciola hepatica EXPUESTA A TRICLABENDAZOLE

GENTILE ML, FELIPE A., SCARCELLA S., ALZOLA R. Y SOLANA H.

Huerta Grande, Córdoba

Congreso; 1ª Reunión Conjunta de Sociedades de Biología de la República Argentina, XVI Jornadas Científicas de la Soc. De Córdoba, IX Jornadas de la Soc. Arg de Biología y XXV Reunión Anual de la Soc. De Biología de Cuyo; 2007

The liver flukes Fasciola hepatica is a trematode that parasites the liver of herbivorous animals and humans, causing severe economic losses. In subtropical and tropical areas its incidence in human is important, affecting mainly suburban populations. Triclabendazole (TCBZ) is used for its treatment. Its  mechanism of action consists in  the despolimerization of F. hepatica microtubules with the consequent loss of function, loosening and death. The aim of the present work was to compare the histopathology of the sperm duct of F. hepatica obtained from untreated parasited bovines (controls) and parasited bovines treated with TCBZ. The sperm ducts of the control individuals showed plenty of cells in different stages of spermatogenesis. In the periphery, spermatogonias I and II were visualized,  and also  basophilic cells  with great nucleus and little cytoplasm. Towards the center of the ducts spermatogonias III were found, also basophilic, with strongly  stained nucleus  and little unstained cytoplasm, spermatocytes (with peripheral nucleus), eosinophilic  cytoplasm and spermatozoa. In liver flukes obtained from the treated individuals the sperm ducts appeared with peripheric vacuolization, disorganization and reduction of the cellular types. These results contribute to the understanding of the pharmacologic activity of this type of drugs.