EXPRESION DE LA GLICOPROTEINA P EN EL TREMATODE Fasciola hepatica

SCARCELLA S.; FELIPE A.; ALZOLA R. Y SOLANA H.

Buenos Aires

Congreso; X Jornadas multidiciplinarias de la Sociedad Argentina de Biologia; 2008

Sociedad Argentina de biologia

Fasciolasis is a parasitic disease produced by the trematode Fasciola hepatica, affecting both human and animals as well. Its treatment is based on the administration of triclabendazole, a benzimidazole. Currently, its indiscriminate use has determined the emergence of resistant strain. Hence, the need to know detoxification and resistant mechanisms in F. hepatica arises. In general, helminths evade antiparasitic effect by: i) mutation of target molecules, ii) overexpression of efflux pumps, i.e. glycoprotein P (pgP), and/or iii) overexpression of metabolic activity. The aim of the present work was to characterize the presence, distribution and expression of pgP in F. hepatica with immunoelectrophoretic and immunohistochemical techniques. Results were confirmed by SDS-PAGE and immunotransfering pgP present in microsomes. Optical microscopical immunohistochemical studies located the pgP mainly in the enterocytes and vitellogenic cells. These studies on protein systems related to drug disposition in parasites are a significant contibution to further understand anthelmintic resistance mechanisms.