FATTY ACIDS FROM Pseudomonas putida A ATCC 12633 INVOLVED IN ADAPTATIVE RESPONSE TO BROMIDETETRADECYLTRIMETHYLAMMONIUM

HEREDIA R.M; LOPÉZ G.A; BOERIS P.S.; LUCCHESI G.I.

Jornada; XIX Annual Scientific Meeting. SOCIEDAD DE BIOLOGÍA DE CÓRDOBA; 2013

P. putida tolerate and degrade high concentrations of cationic surfactant tetradecyltrimethyl ammonium (50 mgl-1) (TTAB).The aim of this work was to establish if the response to TTAB, involves changes in the fatty acid (FA) composition of P. putida. For this, we examined the FA profile of P. putida grown with glucose and NH4Cl exposed or not to TTAB. In absence to surfactant and unlike as described for other Pseudomonas species, P. putida showed predominance of the saturated FA (SFA: 86.86%), having: palmitic acid (16:0, 24%), stearic acid (18:0, 64%) and trace amounts of myristic acid (14:0:1.49%) and heptadecanoic acid (17:0, 2.62%). The remaining fraction was represented by unsaturated FA (UFA): palmitoleic acid (16:1Δ9, 6.29%), cis-oleic acid (18:1cis-Δ9, 1.98%) and cis-vaccenic acid (18:1cis-Δ11, 5.62%). Therefore, the SFA/UFA was 6.22. Respect to untreated cells, in P. putida exposed 15 min to TTAB, the levels the FA 18:0 and the FA 17:0 increased about 10% and 70%, respectively. While the amount of the FA cis-18:1Δ11 and the FA 16:1Δ9 decreased significantly (95%). The SFA/UFA relationship increased 7 fold (reached value 44.32), indicating decreased the degree of fluidity of the cell membrane. Based on the observed differences on the AGS/AGI ratio, we conclude that modifications of FA contents could be part of the rapid response to TTAB, contributing significantly to increase of the rigidity membrane necessary to counteract the effect detergent and to maintain the integrity of the cell envelope.