Response to bromide-tetradecyltrimethylammonium in a P. putida cardiolipina sinthase deficient

LÓPEZ, G.A.; HEREDIA, R.M.; BOERIS, P.S.; LUCCHESI, G.I.

Jornada; II Reunión Conjunta de Sociedades de Biología de la República Argentina; 2011

Pseudomonas putida A ATCC 12633 responds to tetradecyltrimethylammonium (TTAB) through quantitative changes in phospholipids with specific variations in the content of phosphatidic acid, phosphatidylglycerol and cardiolipin. Sequence analysis of P. putida KT 2440 revealed a gene pp5364 as cardiolipin sinthase (cls). The P. putida A ATCC 12633 cls homologue was disrupted to generate the corresponding mutant, P. putida A ATCC 12633 Δcls. In the mutant strain, the cardiolipin levels and distribution into the membranes, visualized using the fluorescent dye 10-N-Nonyl acridine orange, was similar to detected in the wild-type strain, indicating that cardiolipin synthesis occurs by other gene in this organism. The strain was grown on a basal salt liquid medium with glucose and NH4Cl and exposed to 50 mg l-1 of TTAB. The cardiolipin level was similar to the one observed in TTAB absence but the presence of TTAB resulted in an increase in phosphatidylglycerol and phosphatidic acid levels (2 and 10 fold, respectively) with respect to the levels in cells grown without the surfactant, indicating that phosphatidic acid is the primary membrane-associated factor for the response to TTAB.