Biohybrid adsorbent for the preconcentration of lead and its determination in fruit juices by electrothermal atomic absorption spectrometry

PAMELA Y. QUINTAS; ELIZABETH AGOSTINI; ANA WEVAR OLLER; LETICIA B. ESCUDERO

JOURNAL OF AOAC INTERNATIONAL

AOAC INT

Año: 2023

1060-3271

Background: Fruit juices are one of the most non−alcoholic beverages consumed in the world. Essential elements and other nutrients present in fruit juices play an important role in human well−being. However, fruit juices may also contain potentially toxic elements at trace levels, causing health risks. Objective: The objective of this work was to develop an analytical methodology based on the preconcentration of lead using a new biodegradable hybrid material composed of Rhodococcus erythropolis AW3 bacteria and Brassica napus hairy roots. Methods: The biodegradable hybrid material was implemented in an online solid phase extraction system for the determination of lead in fruit juices by electrothermal atomic absorption spectrometry. Results and Discussion: Effects of critical parameters on lead retention were studied. Under optimal experimental conditions, extraction efficiency higher than 99.9% and an enrichment factor of 62.5 were achieved. The dynamic capacity of the biodegradable hybrid material was 36 mg/g, which favored the reuse of the column to at least 8 biosorption−desorption cycles. The limit of detection and quantification for preconcentration of 5 mL of sample were 5.0 and 16.5 ng/L lead, respectively. The relative standard deviation was 4.8% (at 1 µg/L lead and n=10). The developed method was suitable to be applied for lead determination in different types of fruit juices.