Estrogen receptor and endothelial dysfunction

VALLE, MARÍA IVONE; RAUSCHEMBERGER, MARÍA BELÉN; ESPECHE, WALTER; SALAZAR, M; PLUNKETT, RICARDO; MASSHEIMER, VIRGINIA L.

Mar del Plata, Pcia. de Buenos AIres

Congreso; XLIV Reunión Anual SAIC; 2019

SAIC

Estrogen receptor (ER) plays key role on vascular homeostasis. Alterations of ER signaling could conduct to vascular dysfunction, impairment of angiogenesis and platelet aggregation and activation. Preeclampsia (PE) is a pregnancy disease that exhibits these features. We have previously reported that the ER activation by estrone (E1), second natural estrogen, stimulates vasodilators synthesis (NO and PGI2) and inhibits platelet aggregation. This work presents basic (1) and clinical (2) results about the association of ER and vascular dysfunction. 1) Endothelial cells (EC) were incubated with E1 10 nM for 24 h. (2) We tested the existence of association between polymorphisms of ESR1 with PE in a high-risk pregnant women population. Polymorphic variants were studied by RFLP-PCR, employing PvuII and XbaI and resolved by electrophoresis in agarose gels. The in vitro treatment of EC with E1 shows that the hormone stimulated EC growth (91% a/C, p