UPPER AND LOWER AIRWAY INFLAMMATION IN A POLLEN-INDUCED MURINE MODEL OF ALLERGIC RHINITIS

GALVEZ, MARCELO; LENCINAS, ILEANA; GIORGIA, GISELA; CRESCITELLI, M. CARLA; MARTÍNEZ, ADRIANA; MURRAY, GABRIELA; RAUSCHEMBERGER, MARÍA BELÉN; PRAT, MARÍA INÉS

San Luis

Congreso; LXXI Reunión Anual de la Sociedad Argentina de Inmunología; 2023

SAI

More than 80 % of asthmatics have rhinitis and 10-40 % of individuals with rhinitisalso have asthma. Allergic rhinitis (AR) and asthma are frequently associated andoften share therapeutics. Guidelines by World Health Organization emphasizethe importance of links between the upper and lower airways and recognize bothentities as a global airways disease. In our region, Russian thistle (Salsola kali)is a common weed causing pollinosis and previously, our group has used itspollen extract to generate a murine S.kali-specific AR acute model. The aim ofthis study was to establish whether AR may contribute to the inflammatoryresponse of lower airways in a prolonged exposure model. BALB/c mice (n =5/group) were administrated every three days for two weeks with PBS or S. kalipollen extract through i.p. route. Then, mice were challenged by nasal instillationof PBS or S. kali pollen respectively weekly for 6 weeks. After sacrifice, the seraand bronchoalveolar lavage (BAL) were obtained. Total serum IgE and S. kalispecific IgE, IL-4 and IL-5 levels from splenocytes culture were measured byELISA. The noses and lungs were fixed and paraffin embedded for histologicalanalysis (H&E). After nasal challenge, frequency of sneezing in sensitized groupwere higher than in control (p