Similar actions of estrone at bone and vascular level

CRESCITELLI, M. CARLA; RAUSCHEMBERGER, M. BELÉN; MASSHEIMER, VIRGINIA L.

Buenos Aires

Congreso; Congreso; ICE 2021 Virtual: 19th international congress of endocrinology; 2021; 2021

ICE-FASEN

Introduction: It is known that during menopause, the decrease in estrogen levels has a negative impact on boneremodeling and vascular function. Although postmenopausal plasma levels of estradiol are considerably low, those ofestrone (E1) are sustained providing the major source of circulating estrogens. We previously reported that E1 modulatesbone cells growth, and at vascular level the estrogen stimulates cellular events involved in the early steps ofatherosclerosis lesion progression such as, oxidative stress, and platelet and monocyte adhesion. The most advancedstage of atheroma involves vascular calcification (VCa) event triggered by vascular smooth muscle cell (VSMC)transdifferentiation into bone like cells.Objectives: The aim of this work was to evaluate the effect of E1 on VSMC transdifferentiation into osteoblasts (VSMCOB)and to compare it with the E1 action on murine calvarial osteoblasts (OB), focusing our attention on the modulation ofcell maturation.Methods: -Results: E1 showed a mitogenic effect on both cells type, stimulating VSMC-OB and OB proliferation, evaluated by theMTT conversion test (27; 39% a/C p