Nucleic Acids Isolation for Molecular Diagnostics: Present and Future of the Silica-based DNA/RNA Purification Technologies

MIRNA LORENA SANCHEZ; CYNTHIA PEÑALOZA CORONAS; AVILA RODRÍGUEZ, MARÍA ISABELA; PAMELA VIAÑA MENDIETA; HERNANDEZ, GABRIELA RIVERA; JORGE BENAVIDES; MARIANO GRASSELLI

SEPARATION AND PURIFICATION REVIEWS

TAYLOR & FRANCIS INC

Lugar: Londres; Año: 2022

1542-2119

At present, most commercial kits for nucleic acid isolation are silica-based. In this regard, new strategies are under development with the aim of optimizing nucleic acid purification silica-based protocols. This review aims to summarize the evolution of such strategies and highlight the current necessities to be solved by new purification methods based on silica materials. In addition, it will perform a survey of the purified DNA/RNA quality and quantity for a subsequent technique. This information is of vital importance to consider for the success of molecular diagnostics. The text was designed to guide scientists and engineers in choosing suitable DNA/RNA purification techniques for research or bioprocessing applications.