Characterization of Müller glia response after nitro-oleic acid treatment

MARIA VICTORIA VAGLIENTI; MAGALI E. RIDANO; PAULA SUBIRADA; MARIA CONSTANZA PAZ; GUSTAVO BONACCI; MARIA CECILIA SANCHEZ

Congreso; Reunión conjunta SAIC SAI SAFIS 2018; 2018

Proliferative retinopathies are among leading causes of irreversible blindness. Inflammation, oxidative and nitrosative stress are involved in the pathogenesis of diabetic complications, including retinopathy. Although, vascular endothelial growth factor (VEGF) inhibitors have been established as the mainstay of current treatment, the clinical benefits have not always been successful in preserving retinal function. In this regard, we hypothesized that Keap1/Nrf2 pathway can modulate the antioxidant response in neovascular retinopathies. For this reason, we used the activating Keap1/Nrf2 nitro-fatty acids (NO2-FA), which are important electrophilic signaling mediators with anti-inflammatory and cytoprotective activities. Due to Müller Glial cells (MGCs) are commanding survival and death in retina, we studied the effect of nitro-oleic acid (NO2-OA) in the human MGC line, MIO-M1. Initially, cell viability was assessed by MTT assays. MIO-M1 cells exposed to 0,1, 1, 2,5, 5 or 10 μM NO2-OA during 24 to 72 h showed no significant reduction in cell viability (p