SURVIVIN AS A MODULATOR OF AUTOPHAGY AND SENESCENSE IN PANCREATIC ADENOCARCINOMA CELL LINES

MIHALEZ C.Y.; DÍAZ M.; COSTANTINO S.; LEVERMANN M.; LOMBARDO T.; PIBUEL M.; LOMPARDÍA S. L.; PAPADEMETRIO D. L.; ÁLVAREZ É.

CABA

Congreso; Reunión Conjunta de Sociedades de Biociencias; 2017

SAI, SAIC, SAH, SAP, SAIB, SAA, SAB, SAB, SAFE y SAFIS

Pancreatic tumors are highly resistant to chemotherapy. The use of Gemcitabine (Gem) raises the levels of survivin expression and this is accompanied by increases in basal autophagy levels.Recently, it has been shown that exist a link between the processes of autophagy and senescence. Therefore, we proposed to evaluate whether survivin would be mediating these biological processes in our cell lines. For this purpose, we treated the lines MIAPaCa-2 wt and Sur -/- with Gem (10, 100 and 1000 μg/ml) alone or in combination with 3MA for 48 hs and determined the percentage of SA-β-Gal positive cells, to evaluate senescence. On the other hand, we treated the cell lines with Gem (10, 100 and 1000 µg/ml) alone or in combination with 3MA for 48 hs to determine the percentage of apoptosis by TUNEL assay. We could observe in the wt line that the treatment with Gem increases the levels of senescence in a dose-dependent manner reaching values of 80% (p