ROLE OF HYALURONAN (HA) AND ITS RECEPTOR RHAMM IN PROLIFERATION, MIGRATION AND DIFFERENTIATION USING JEG-3 CHORIOCARCINOMA CELL LINE. CHARACTERIZATION OF ENZYMES RELATED WHIT HA METABOLISMS

PIBUEL M; MASCARÓ M.; DÍAZ M.; LOMPARDÍA S. L.; MIHALEZ C.Y.; PAPADEMETRIO D. L.; LEVERMANN M.; ALVAREZ E. M. C.; HAJOS S. E.

Mar del Plata

Congreso; Reunión Científica Anual de SAIC; 2016

SAIC

Choriocarcinoma (CC) is an aggressive neoplasia that affectswomen in fertile age. Although molecular aspects of this pathologyhave been studied those related with extracellular matrix have notbeen totally clarified. The aim of this work was to evaluate theexpression and involvement of HA and RHAMM in CC biology. HumanJAR, JEG-3, BeWo and rat Rcho CC cell lines were used. HAsecretion was measured by ELISA-like assay. RHAMM expressionwas determined by western blot and IFI. RHAMM, HA synthases(HAS) and hyaluronidases (Hyals) mRNA expression were evaluatedby RT-PCR. The effect of several molecular weight HA (high ,low, and oligomers) in migration, proliferation and differentiation(HLA-G and Syncitin-2 mRNA expression) was determined bywound healing and transwell assays, [3H]-thymidine incorporationand RT-PCR, respectively. Ly294002 (PI3K inhibitor) and UO126(MEK1/2 inhibitor) were used to evaluate signalling pathways involvedusing JEG-3 cell line. All cell lines studied showed HAS 1-2and Hyals1-2 expression while only BeWo showed higher basalsecretion of HA respect to its cell-free medium (p