FABP1 REGULATES GENE EXPRESSION IN COLORECTAL CANCER CELLS

BORUS, D; ZADRA, G; MINSKY, D; RODENAK KLADNIEW B; CÓRSICO, B; SCAGLIA, N

Mar del Plata

Congreso; REUNIÓN CONJUNTA SAIC SAI&FAIC SAFIS 2022; 2022

SAIC SAI SAFIS

Fatty acid binding proteins (FABPs) are small cytosolic proteins thatreversibly bind fatty acids (FA) and other lipophilic compounds. Inhumans, ten isoforms have been reported with both overlapping anddistinct expression patterns, suggesting differential functions. TwoFABPs isoforms are abundant in intestinal epithelial cells: the liverFABP (FABP1) and the intestinal FABP (FABP2). They are thoughtto be associated with intracellular dietary lipid transport and trafficking towards diverse cellular fates. Their specific functions are,however, still poorly understood. Given the importance of dietaryfats in colorectal cancer (CRC) development, we analyzed the roleof FABP1 in CRC lipid metabolism. To this end, we used Caco-2cells, a well-established model of human CRC, to generate a stableFABP1 knockdown model using an antisense strategy (named Caco2-FABP1as cells). We have previously shown that FABP1 knockdown resulted in extensive changes in lipid metabolism, includinga diminished incorporation of exogenous FA into complex lipids,particularly in the phospholipids class. Accordingly, FABP1as cellsexhibited a dramatic decrease in proliferation rate. Here we show that Caco2-FABP1as cells have decreased de novo FA synthesis(p