Effect of Fatty Acids on the secretion of proinflammatory cytokines in intestinal epithelial cells.

RODRIGUEZ SAWICKI LUCIANA; FALOMIR LOCKHART LISANDRO JORGE; BOTTASSO NATALIA; CHIRDO FERNANDO; CÓRSICO BETINA

Puerto Madryn

Congreso; XLVI Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular (SAIB); 2010

Sociedad Argentina de Investigación en Bioquímica y Biología Molecular (SAIB)

Intestinal epithelial cells (IEC) form the first line of defense against microorganisms and food derived antigens contained in the intestinal lumen. As such, IEC also participates in the assimilation and processing of a large quantity of lipids incorporated with the diet . These cells express Intestinal Fatty Acid Binding Protein (IFABP) and Liver-FABP. The classical functions attributed to these proteins are as cytosolic buffers and transporters of hydrophobic ligands. Nevertheless, new insigths have been suggested, FABP could have a role in the regulation of lipid metabolism and other cellular processes. In this work we planned to study the effect of principal dietary fatty acids on the secretion of proinflammatory cytokines in the Caco-2 cell intestinal epithelial model. The role of l- and LFABP will be also assessed using this model. We analyzed this effect by evaluation of mRNA expression level of cytokines such as IL6, IL8, CCL2 and CXCL10 by Real Time-PCR. Preliminary results suggest that oleic and palmitic acid would be increasing mRNA cytokines levels in a time dependent manner. What is more, the analysis of the effect of these fatty acids in the secretion of proinflammatory cytokines using the Caco-2 LFABP knock down model previously obtained in our laboratory will contribute to the knowledge of new functions for this proteins involved in the immunity of IEC.