A cellular model to study the effect of prolactin on human endometrium

ANAHÍ FRANCHI; DIEGO UÑATES; LAURA C. GIOJALAS

Ciudad Autónoma de Buenos Aires

Jornada; XII Jornadas de la Sociedad Argentina de Biología; 2010

Sociedad Argentina de Biología

Introduction. The expression of prolactin (PRL) and its receptor (PRL-R) increase during endometrial decidualization. However PRL role in the endometrium is not known. Previous results in endometrial epithelial cells (EEC) primary cultures have shown that PRL induce an increased expression of MFG-E8 protein (?milk fat globule EGF-factor 8?), supporting the hormone possible role on endometrial differentiation. Objectives. Characterize an established cell line system as a model to study PRL role on human endometrium. Methodology. The presence of PRL-R was analyzed by Western blotting (WB) and indirect immunofluorescence (IIF) on Ishikawa and HEC-1A endometrial epithelial cell lines. Results. The presence of two PRL-R isoforms was detected by WB. Both cell lines showed bands of 80 and 50 kDa corresponding to the long and intermediate receptor isoformas respectively. The IIF showed an intracytoplasmic staining, stronger at the peri-nuclear region of the cells. Discussion. The use of established cell lines represent and advantage compared to primary cultures. The expression of PRL-R in EEC lines indicates these cell lines would be a valuable tool for studying PRL role in human endometrium.