Correlation between StarD7, B-catenin and Inos transcript levels in rat tissues

FLORES MARTIN J; RENA VC; PANZETTA DUTARI G; GENTI RAIMONDI S

Villa Carlos Paz, Cordoba. Argentina

Congreso; XLIV Reunion Anual de la Sociedad Argentina de Investigacion en Bioquimica y Biologia Molecular (SAIB); 2008

SAIB

StarD7 transcript encodes one of the 15 family members with START domain present in the human genome. Highly similar human StarD7 orthologous genes have been annotated in GenBank databases in different genomes, suggesting a conserved physiological function. We demonstrated that StarD7 gene expression in human cells is regulated by Wnt/β-catenin signaling which is implicated in numerous aspects of development, cell biology and physiology such as proliferation, inflammation, and cancer. The aim of the present study was to evaluate StarD7 transcript expression in rat tissue samples and correlate them with β-catenin and inducible oxide nitric synthase (iNOS) ones, which is also target of β-catenin pathway. RT-PCR techniques for StarD7,β-catenin and iNOS were performed in liver, brain and ovary isolated from normal rats. The transcripts were detected in all tissue samples although the StarD7 tissue distribution was 2-4 times elevated in brain compared to ovary and 60-80 times higher in liver than ovary. These data show a significant correlation between expression of StarD7 and β-catenin mRNA level as well as with iNOS expression and set more evidences indicating that StarD7 gene is a target of Wnt/β-catenin signaling pathway. (Presentacion Poster)