StarD7 siRNA modulates ABCG2 expression, cell migration and proliferation in trophoblast cell lines

FLORES MARTIN J; RENA VC; PANZETTA DUTARI G; GENTI RAIMONDI S

Foz do Iguazu, PR

Congreso; V-SLIMP (Sociedad Latinoamericana de Interacción Materno-Fetal y Placenta) y IV-LASRI (Latin American Society for Reproductive Inmunology); 2013

Sociedad Latinoamericana de Interacción Materno-Fetal y Placenta

StAR-related lipid transfer domain containing 7 (StarD7) belongs to the family of START proteins ubiquitously expressed implicated in lipid transport, metabolism, and signaling. Previous results indicate that StarD7 silencing decreases ABCG2 expression, cell migration, proliferation, and reduction in phospholipid synthesis; whereas an increase in the biochemical and morphological differentiation marker expression was detected in JEG-3 cells. In order to extend these findings to other trophoblast cells, we performed StarD7 knockdown in HTR8/SVneo and BeWo cells. StarD7 silencing, confirmed by qRT-PCR and western blot, led to a decrease in the xenobiotic/ lipid transporter ABCG2 at both the mRNA and protein levels. Increased hCG mRNA was detected in BeWo cell line. Wound healing and transwell assays revealed that HTR8/SVneo cell migration was significantly diminished. Altogether these data provide evidence for a StarD7 conserved function in cell physiology. Current work is in progress to establish the mechanisms involved in these findings.