Biological samples analysis using phases contrast radiography

ROCHA, H.S.; LOPES, R.T.; HÖNNICKE, M.G.; TIRAO, G.; MAZZARO, I.; C. CUSATIS; GILES, C.; PESSÔA, L.M.

Campinas

Congreso; XIII Reunión Anual de Usuarios del LNLS; 2003

LNLS

The fundaments of phase contrast theory arise in 1948 with the Garbor´s article[1] about new principles in microscopy. Years later, Cosslett and Nixon[2] published the first work with the principles of the x-ray for phase contrast images (PCI). A conventional x-ray is obtainedthrough the interaction of an X-ray incident beam with an object. This interaction causes attenuation in the emergent beam and the formed image is a map of intensity attenuation of the object. The technique of phase contrast is based on refraction phenomena, diffractionand scattering (rejection) where, starting from a special geometry, the change of phase of a wave can be observed after crossing an object. The phase change cannot be observed pure and simply, however its effects contribute to increase the image contrast since the number of photonsgrows in a certain area. This work presents the contrast phase imaging technique evaluating the possibility of studying pathological human samples and pattern recognition in biological species[3].