INVESTIGADORES
COLLINS Sebastian Enrique
capítulos de libros
Título:
Modulated Infrared Spectroscopy for Understanding Enzymes at Work
Autor/es:
BRIAND, LAURA; COLLINS, SEBASTIÁN E.
Libro:
Springer Handbook of Advanced Catalyst Characterization
Editorial:
Springer
Referencias:
Año: 2023; p. 1 - 18
Resumen:
In this case of study, we provide examples of the use of infrared spectroscopy in total attenuated reflection (ATR) to investigate enzymes at work. An optimized ATR flow-thought cell was developed, which allows performing modulation excitation spectroscopy with phase sensitive detection (MES-PSD) experiments in a controlled way. The reversible effect of solvents with different hydrophobicity on the secondary structure of a Thermomyces lanuginosus lipase (TTL) was demonstrated by an analysis of the Amide I band. Finally, the lipase B of Candida antarctica (CALB), active to the enantioselective esterification of ketoprofen with alcohols was studied. MES-PSD analysis allowed to univocally identifying signals belonging to the interaction between ketoprofen and the lipase, that is the acyl enzyme intermediate.
