INVESTIGADORES
OTTADO Jorgelina
congresos y reuniones científicas
Título:
AN ACQUIRED BACTERIAL PLANT NATRIURETIC PEPTIDE-LIKE PROTEIN MODIFIES HOST HOMEOSTASIS
Autor/es:
GOTTIG, N.; GARAVAGLIA, B.S.; DAURELIO, L. D.; ORELLANO, E; JORGELINA OTTADO
Lugar:
Villa Carlos Paz
Reunión:
Congreso; XLIV Reunión Anual de la Sociedad Argentina de Bioquímica y Biología Molecular; 2008
Resumen:
Plant natriuretic peptides (PNPs) are a class of extracellular,
systemically mobile peptides that elicit a number of plant responses
important in homeostasis and growth. The bacterial citrus pathogen
Xanthomonas axonopodis pv. citri (Xac) also contains a gene
encoding a PNP-like protein, XacPNP, that shares significant
sequence similarity and identical domain organization with PNPs
but has no homologues in other bacteria. We have expressed and
purified XacPNP and demonstrated that the bacterial protein, like
PNPs, could alter plant physiological responses. We also observed
that XacPNP transcription can respond to the host environment
suggesting a role during bacterial infection. To characterize the role
of XacPNP in citrus canker, we constructed a XacPNP deletion
mutant. The lesions caused by this mutant were more necrotic than
those observed with the wild-type and bacterial cell death occurred
earlier in the mutant. Moreover, when we expressed XacPNP in
Xanthomonas axonopodis pv. vesicatoria, the transgenic bacteria
caused less necrotic lesions in the host than the wild-type. We have
evidence to conclude that XacPNP enables Xac to modify host
homeostasis in order to create conditions favorable to its own
survival