Outer membrane vesicles obtained fromBordetella pertussisTohama expressing the lipid A deacylase PagL as a novel acellular vaccine candidate

ASENSIO, CRISTIAN; GAILLARD, M. EMILIA; MORENO, GRISELDA; BOTTERO, DANIELA; ZURITA, MARIA EUGENIA; RUMBO, MARTIN; VAN DER LEY, PETER; VAN DER ARK, ARNO; HOZBOR, DANIELA

VACCINE

ELSEVIER SCI LTD

Año: 2011 vol. 29 p. 1649 - 1656

0264-410X

In an effort to devise a safer and effective pertussis acelullar vaccine, outer membrane vesicles (OMVs) were engineered to decrease their endotoxicity. ThepagL gene fromBordetella bronchiseptica, which encodes a lipid A 3-deacylase, was expressed inBordetella pertussisstrain Tohama I. The resulting OMVs, designated OMVsBpPagL, contain tetra- instead of penta-acylated LOS, in addition to pertussis surface immunogens such as pertactin and pertussis toxin, as the wild type OMVs. The characterized pertussis OMVsBpPagLwere used in murineB. pertussisintranasal (i.n.) challenge model to examine their protec-tive capacity when delivered by i.n. routes. Immunized BALB/c mice were challenged with sublethal doses ofB. pertussis. Significant differences between immunized animals and the PBS treated group were observed (p