HYPOTHYROIDISM ALTERS PI3K/AKT SIGNALING DURING LACTATION

FIORELLA CAMPO VERDE ARBOCCO; PERSIA ANDRES; HAPON, MARÍA BELÉN; CARON RUBEN; JAHN GA

Buenos Aires

Congreso; LXII ANNUAL MEETING OF ARGENTINE SOCIETY OF CLINICAL INVESTIGATION; 2017

SAIC

Lactation is key in mammary differentiation and is regulated byprolactin and the JAK2/STAT5 and AKT signaling pathways. It isknown that hypothyroidism (hypoT) has deleterious effects on theJAK2/STAT5 pathway. In this work, we evaluated the impact ofPTU-induced hypoT on lactating rat mammary AKT pathway, whichhas been intimately linked with mammary cellular differentiation,transcriptional and translational cell performance and mammary pathologieslike cancer. We evaluated the expression level and phosphorylationof the three AKT isoforms and the two PI3K sub-units bywestern blot in the mammary glands (n = 6) from rats on lactationdays 2, 7 and 14 (L2, L7 and L14). Results were analyzed by twowayANOVA and Bonferroni post-test. We found that on L7 hypoTdecreased the expression level of the p85 subunit of PI3K (p