Tessaria absinthioides INDUCES TRH LIKE RESPONSE IN FEMALE BALB/C MICE

PENAS, VERONICA; ESTEFANÍA RINALDINI; CARLOS GAMARRA LUQUES; HAPON M. BELÉN

Villa de Merlo

Congreso; Sociedad de Biología de Cuyo 2017; 2017

Sociedad de Biologia de Cuyo

Tessaria absinthioides (HOOK & ARN) DC. (Ta) is a native plant from Mendoza ? Argentina with reported ethnopharmacological and culinary uses. Because it?s promissory activity as cytotoxic and anti-tumoral compound, our group started to research about toxic effects in animals, when the aqueous extract is orally administrated at doses of 300 mg/animal/day. Earlier studies discard acute and sub-chronic toxicity in adults Sprague Dawley males and females. Therefore, reproductive toxicity and pre/post-natal exposure were studied in BALB/c mice; none deleterious effects were observed in mature or pregnant females; nor born or lactation pups. The goal of the current work is to describe the status of hypothalamus ? hypophysis ? thyroid axis to analyze the capability of Ta extract to act as endocrine disruptor. So, serums of treated female BALB/c mice were analyzed to determine TSH, T4 and T3 levels; by enzyme linked immunosorbent assay and radioimmunoassay. Moreover, Thyroid morphological features were histologically evidenced. Results demonstrated a no significant increases in TSH into treated group respect to controls; accompanied by a significant increase in T4 levels (Student´s T test, p=0.04), without changes in T3 concentration. Microscopically, gland acini show signs of hyperactivity with enlarged cubic cells containingprominent nucleus, and increased thyroglobulin resorptive spaces. In accordance to these evidences, it is possible to affirm that some natural derivatives act as ?TRH like? compound inducing the hypophysis response by activation of specific TRH receptors. In conclusion, it is not possible to dismiss endocrine disruption as consequence of Ta oral intake.