EVALUATION OF CYTOTOXIC EFFECT OF Tessaria absinthioides DIFFERENT HARVESTS AND AQUEOUS PREPARATIONS ON p53 +/- HUMAN BREAST CANCER CELL LINES

PASCUAL, LOURDES; SANMARTINO JULIAN; HAPON M BELEN; GAMARRA-LUQUES, CARLOS

Mendoza

Congreso; XL Reunión Científica Anual de la Sociedad de Biología de Cuyo; 2022

Sociedad de Biologia de Cuyo

According to the Global Cancer Observatory, breast cancer was the most prevalent in Argentina in the year 2020, with 22,024 reportedcases, representing 16.8% of all the new cases and being the most frequent in women (32.1%), with the highest mortality (19.9%). Thisdata shows the need to improve current treatments, not only to increase their effectiveness but also to reduce their side effects. Newstrategies are being explored by incorporating natural compounds as plant-derived products that might be beneficial in benefit cancermanagement. The aqueous extract of T. absinthioides (AETa) has been reported for its cytotoxicity against cancer cell lines, with no-toxicand antitumoral effects when it is orally administered in animals. The aim of this study was This study aimed to compare the cytotoxiceffects of T. absinthioides different harvests and aqueous preparations on MCF7 (p53 +) and T47D (p53 -, mutated) cell lines. Plant leavesharvested in 2017, 2018 and 2019 (Lavalle County, Mendoza) were used to prepare the AETa by boiling 10 g leaves in 100 mL water,followed by heat concentration up to the final volume of 10 mL. The decoction (DETa) was prepared by boiling of 10 g leaves in water(100 mL), without later concentration. Finally, to prepare the infusion (INTa), 10 g of leaves were placed in 100 mL of hot water (96°C)for 10 minutes. In order to investigate the cytotoxic activity of different harvests and preparations on MCF-7 and T47D cell lines, the cellproliferation was assessed by performing an MTT assay in a dose-response experimental design. The treatments were applied in the culturemedia for 72 h, and their doses were estimated by the dry content of leaves in the solution. The treatments potency was calculated by demedian effective dose (Dm, as the concentration that inhibits proliferation at 50%), using CompuSyn software. This study demonstratedthat the cytotoxic activity of AETa on MCF-7 and T47D cells did not vary significantly between the 2017, 2018 and 2019 harvests. InMCF7, Dm values were 9.86, 9.99 and 7.60 μg/mL, respectively; while T47D cells showed Dm values of 13.84, 13.79 and 13.07 μg/mL.Then, when the effect between different preparations were analyzed, AETa and DETa showed a comparable activity on both cell l ines(MCF-7 Dm = 7.99 and 6.81 μg/mL; and T47D Dm = 9.47 and 9.13 μg/mL). In contrast, INTa showed a significantly decreased cytotoxiceffect (ANOVA followed by Tukey´s multiple comparisons test, p < 0.05) on both cell lines (29.75 and 35.32 μg/mL, respectively). Later,when obtained Dm where were compared between both cell lines, the MCF-7 cells were always significantly more susceptible to thetreatments than the T47D cells (Student T test, p < 0.05). These results demonstrate: first, that the plant activity resulted similar similarlywhen harvests from different years were compared; second, that the decoction resulted in the optimum process for the plant aqueous plantpreparation; and finally, that the p53 protein expression sensitizes the breast cancer cells to T. absinthioides treatment. In conclusion, thisstudy represents a valuable evidence of T. absinthioides aqueous extract activity, promoting its application in oncology research.