Cell fate of retinal progenitor cells: In ovo UbC-SarTrack analysis

OLMOS-CARREÑO, CINDY LORENA; FIGUERES OÑATE, MARÍA; SCICOLONE, GABRIEL; LÓPEZ-MASCARAQUE, LAURA

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

MOLECULAR DIVERSITY PRESERVATION INTERNATIONAL-MDPI

Lugar: Basel; Año: 2022 vol. 23 p. 1 - 16

1422-0067

Clonal cell analysis outlines the ontogenic potential of single progenitor cells, allowing theelucidation of the neural heterogeneity among different cell types and their lineages. In this work,we analyze the potency of retinal stem/progenitor cells through development using the chick embryo as a model. We implemented in ovo the clonal genetic tracing strategy UbC-StarTrack for tracking retinal cell lineages derived from individual progenitors of the ciliary margin at E3.5 (HH21-22).The clonal assignment of the derived-cell progeny was performed in the neural retina at E11.5-12(HH38) through the identification of sibling cells as cells expressing the same combination of fluorophores. Moreover, cell types were assessed based on their cellular morpholog and laminar location. Ciliary margin derived-cell progenies are organized in columnar associations distributed along the peripheral retina with a limited tangential dispersion. The analysis revealed that, at the earlystages of development, this region harbors multipotent and committed progenitor cells.