COMPOUNDS WITH POTENTIAL PRO-HEALING AND ANTI-PATHOGENIC PROPERTIES IN SKIN CHRONIC WOUNDS. CHEMICAL AND BIOLOGICAL CHARACTERIZATION OF LACTOBACILLUS PLANTARUM CULTURE SUPERNATANS

ALBERTO N. RAMOS; MARÍA E. SESTO CABRAL; MARIO EDUARDO ARENA; FEDERICO ARRIGHI; ABEL ARROYO AGUILAR; JUAN CARLOS VALDEZ

PHARMACEUTICAL BIOLOGY

TAYLOR & FRANCIS INC

Lugar: Londres; Año: 2014

1388-0209

Context: It is necessary to advance the field of alternative treatments for chronic wounds that are financially accessible to the least economically developed countries. Previously we demonstrated that topical applications of Lactobacillus plantarum cultures supernatants (LAPS) on human infected chronic wounds reduce the pathogenic bacterial load, the amount of necrotic tissue, and the wound area, as well as promote debridement, granulation tissue, and wound healing. Objective: To study LAPS chemically and biologically, to find potential molecules responsible for its pro-healing properties in chronic wounds. Materials and methods: 1) Chemical analysis: Extracts were subjected to a column chromatography and the concentrated fractions obtained were studied by GCMS. 2) Quantification: DL-acid lactic (Commercial kit), Phenolic compounds (Folin Ciocalteu), H2O2 (micro-titration), cations (Flame photometry). 3) Biological analysis: Autoinducers type 2 (AI-2) (Vibrio harveyi BB170 bioassay), DNAase activity (Agar DNAase), Pseudomonas aeruginosa biofilm inhibition (crystal violet technique). Results: According to its biological activity, the most significant molecules found by GCMS were: Antimicrobials (Mevalonolactone, 5 methyl hydantoine, benzoic acid and others); surfactants (di-palmitin, distearin, 1,5 monolinolein); anesthetics (barbituric acid derivatives) and AI-2 precursors (4,5-dihydroxy-2,3-pentanedione and 2-methyl-2,3,3,4-tetrahydroxytetrahydrofurane). Concentrations measured (µg/mL): DL-lactic acid (11.71 ± 1.53); H2O2 (36 ± 2.0); Phenolic compounds (485.2 ± 15.20); sodium (370 ± 17); potassium 920 ± 24); calcium (20 ± 4); magnesium (15 ± 3). DNAase from LAPS, had activity on genomic DNA from PMNs and P. aeruginosa. Discussion and Conclusion: The molecules and biological activities found in LAPS, could explain the observed effects in human chronic wounds.