INVESTIGADORES
PISTORIO Mariano
artículos
Título:
First genomic analysis of the broad-host-range Rhizobium sp. LPU83 strain, a member of the low-genetic diversity Oregon-like Rhizobium sp. group
Autor/es:
GONZALO A. TORRES TEJERIZO; MARÍA FLORENCIA DEL PAPA; WALTER O. DRAGHI; MAURICIO J. LOZANO; MARÍA DE LOS ANGELES GIUSTI; CARLA MARTINI; EUGENIA SALAS; ILEANA SALTO; DANIEL WIBBERG; RAFAEL SZCZEPANOWSKI; STEFAN WEIDNER; ANDREAS SCHLÜTER; ANTONIO LAGARES; MARIANO PISTORIO
Revista:
JOURNAL OF BIOTECHNOLOGY
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Año: 2011 vol. 155 p. 3 - 10
ISSN:
0168-1656
Resumen:
Abstract: Alfalfa (Medicago sativa) is the most cultivated forage legume for cattle and animal feeding,
occupying about 32 million hectares over the world. Management of the N2-fixing symbiosis of this
plant to maximize crop production is therefore an important objective. A fundamental constraint to
this aim emerges when a moderately low soil pH hampers the establishment of an effective symbiosis
with indigenous and/or inoculated rhizobia. Besides the association of alfalfa with Ensifer
(Sinorhizobium) meliloti, this legume is able to establish a symbiosis with Ensifer (Sinorhizobium)
medicae and with less characterized types of rhizobia, such as the Oregon-like strains, Rhizobium sp.
Or191 initially isolated in the USA, and the Rhizobium sp. LPU83 strain, from Argentina. These strains
are acid-tolerant, highly competitive for acidic-soil-alfalfa nodulation, but inefficient for biological
nitrogen fixation with alfalfa. These features position the Oregon-like rhizobia as strains of potential
risk in agricultural soils compared with the efficient symbiont E. meliloti. Moreover, the collected
genetic information has revealed that the genomic structure of these rhizobial isolates is complex in
terms of sequence similarities shared with other rhizobia. Such a "patched" genetic composition has
obviously imposed severe restrictions to the classical taxonomy of these rhizobia. In this review we
summarize the accumulated knowledge about the Oregon-like rhizobia and present a phylogenetic
analysis based on genome sequence data of Rhizobium sp. LPU83 obtained by a high-througthput
sequencing on the Genome Sequencer FLX Titanium platform. The accessibility of the complete
genomic sequence will release up more experimental possibilities since this information will then
enable biochemical studies as well as proteomics and transcriptomics approaches.