INVESTIGADORES
PISTORIO Mariano
artículos
Título:
Novel tnpR-based transposable promoter traps suitable for RIVET studies in different gram-negative bacteria
Autor/es:
LOZANO, M. J.; SALAS, M, E.; GIUSTI, M. A.; MARTINI, C; LÓPEZ, J. L.; SALTO, I. P.; DEL PAPA, M.F.; PISTORIO, M.; LAGARES, A.
Revista:
JOURNAL OF MICROBIOLOGICAL METHODS
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Lugar: Amsterdam; Año: 2013 vol. 93 p. 9 - 11
ISSN:
0167-7012
Resumen:
The preparation of plasmid-borne RIVET libraries can be troublesomewhen high genomic coverages are needed. We present here the construction and functional validation of a new set of miniTn5 promoter traps to generate tnpR-based RIVET libraries. The ability to generate tnpR transcriptional fusions by transposition will ignificantly facilitate the setup of RIVET studies in those bacteria where Tn5 transposition is operative.
