Colloidal properties of sodium caseinate-stabilized nanoemulsions prepared by a combination of a high-energy homogenization and evaporative ripening methods

MONTES DE OCA-ÁVALOS, J.M.; CANDAL, R.J.; HERRERA, M.L.

FOOD RESEARCH INTERNATIONAL

ELSEVIER SCIENCE BV

Año: 2017

0963-9969

Nanoemulsions stabilized by sodium caseinate (NaCas) were prepared using a combination of a high-energyhomogenization and evaporative ripening methods. The effects of protein concentration and sucrose addition onphysical properties were analyzed by dynamic light scattering (DLS), Turbiscan analysis, confocal laser scanningmicroscopy (CLSM) and small angle X-ray scattering (SAXS). Droplets sizes were smaller (~100 nm in diameter)than the ones obtained by other methods (200 to 2000 nm in diameter). The stability behavior was also different.These emulsions were not destabilized by creaming. As droplets were so small, gravitational forces were negligible.On the contrary, when they showed destabilization the main mechanism was flocculation. Stability ofnanoemulsions increased with increasing protein concentrations. Nanoemulsions with 3 or 4 wt% NaCas wereslightly turbid systems that remained stable for at least two months. According to SAXS and Turbiscan results,aggregates remained in the nano range showing small tendency to aggregation. In those systems, interactiveforces were weak due to the small diameter of flocs