Discriminating of Foot-And-Mouth Disease Virus-Infected And Vaccinated Animals Through Beta-Galactosidase Allosteric Biosensors

MARÍA TERESA SANCHEZ; MARÍA FLORA ROSAS; ROSA MARIA FERRAZ; LAURA DELGUI; ESTHER BLANCO; ANTONIO VILLAVERDE; FRANCISCO SOBRINO

CLINICAL AND VACCINE IMMUNOLOGY

American Society of Microbiology

Lugar: Washington; Año: 2009 vol. 16 p. 1228 - 1235

1556-6811

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mso-level-number-position:left; margin-left:264.0pt; text-indent:-18.0pt;} @list l2:level8 {mso-level-number-format:alpha-lower; mso-level-tab-stop:300.0pt; mso-level-number-position:left; margin-left:300.0pt; text-indent:-18.0pt;} @list l2:level9 {mso-level-number-format:roman-lower; mso-level-tab-stop:336.0pt; mso-level-number-position:right; margin-left:336.0pt; text-indent:-9.0pt;} ol {margin-bottom:0cm;} ul {margin-bottom:0cm;} --> β -galactosidases accommodating one or two different peptides from the foot-and-mouth disease virus (FMDV) nonstructural protein 3B per enzyme monomer showed a drastic enzymatic activity reduction, which mainly affected proteins with double insertions. Recombinant β -galactosidases were enzymatically reactivated by 3B-specific murine monoclonal and rabbit polyclonal antibodies. Interestingly, these recombinant β-galactosidases, particularly those including one copy of each of the two 3B sequences, were efficiently reactivated by sera from infected pigs. We found reaction conditions that allowed differentiation between sera of FMDV-infected pigs, cattle, and sheep and those of naïve and conventionally vaccinated animals. These FMDV infection-specific biosensors can provide an effective and versatile alternative for the serological distinction of FMDV-infected animals.