INVESTIGADORES
WEVAR OLLER Ana Laura
artículos
Título:
Effects of arsenate on tobacco hairy root and seedling growth, and its removal
Autor/es:
MELINA A. TALANO & ANA L. WEVAR OLLER & PATRICIA GONZÁLEZ & SOLEDAD OLIVA GONZÁLEZ Y ELIZABETH AGOSTINI
Revista:
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY. PLANT
Editorial:
SPRINGER
Referencias:
Lugar: Berlin; Año: 2013 p. 217 - 225
ISSN:
1054-5476
Resumen:
Arsenic (As) is a highly toxic environmental contaminant
to which most living organisms are exposed. Plants
have evolved several mechanisms to cope with this toxic
metalloid; however, these mechanisms are only partially understood.
The response of plants to As phytotoxicity is highly
complex, with considerable variation among species. In this
study, arsenate (As+5) effects on germination and early root
development of tobacco (Nicotiana tabacum) seedlings were
investigated. Also, As+5 tolerance and removal efficiency of
tobacco hairy roots (HRs) and seedlings were assessed and
compared. Total seed germination capacity was not affected
by 10 to 200 μM As+5, while primary root length and root
branching were reduced by As+5 concentrations that were at or
above 100 μM. Both systems were able to tolerate As+5
concentrations of 10 μM since no growth inhibition was
detected. For higher As+5 concentrations, phytotoxicity increased,
but it was mitigated by higher phosphate (Pi) availability.
Under the studied conditions, As+5 removal efficiency
of HRs greatly exceeded that of seedlings. Further, tobacco
HRs were able to accumulate As in their tissues. These results
justify further investigations on As tolerance and detoxification
mechanisms in tobacco, an easy-to-transform crop species
with high biomass, which could allow evaluation of the
possible application of wild type or alternatively transgenic
tobacco plants for As phytoextraction.