NONYLPHENOL INDUCES CYTOSKELETAL CHANGES AND RELEASE OF PROINFLAMMATORY MEDIATORS IN RAT SERTOLI CELLS IN VITRO

VALLÉS, A.S.; GODOY-SEPÚLVEDA, R.; ORESTI, G.M.; MORENO, R.D.; AVELDAÑO, M.I.; REYES, J.G.

Congreso; REUNIÓN CONJUNTA DE SOCIEDADES DE BIOCIENCIAS; 2017

Nonylphenol (NP), an alkylphenol present in plasticizers, is an endocrinedisrupting chemical that is potentially dangerous for malereproduction in mammals including man. Because Sertoli cells (SC)provide structural and metabolic support to germ cells, in this studythe hypothesis that exposures of SC to NP affect their metabolicfunctions and the production of bioactive molecules was evaluated.Primary cultures of SC were preincubated with [3H]arachidonicacid (AA) to label their lipids and then were treated with NP. The NPexposures resulted in increased concentrations of free AA in cellsand medium, indicating that such AA was released from [3H]-labeledlipids (p