INVESTIGADORES
ORESTI Gerardo Martin
congresos y reuniones científicas
Título:
Heat stress affects the metabolism of lipids of epididymal epithelial cells
Autor/es:
LUQUEZ, J.M.; ORESTI, G.M.; VALLÉS, A.S.; AVELDAÑO, M.I.; FURLAND, N.E.
Lugar:
ROSARIO
Reunión:
Congreso; L Reunión Nacional de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular (SAIB); 2014
Resumen:
Frequent
exposures of testes to hyperthermia are known to result in germ cell death,
and, although not lethal to Sertoli cells, we previously showed that such
exposures damage them structurally and functionally. Here we studied the
consequences of heat stress on the epididymis by assessing its effects on
epithelial cells from its three regions (caput, corpus, and cauda) in primary
culture. All of them had abundant lipid droplets (LD) rich in triacylglycerols
(TAG), similar in size and numbers in vivo and in culture. Temperature-treated
(a 15 min exposure once a day to 43ºC for 5 days) and control cells were
incubated for 1 h with [3H]20:4 on the 5th day. In the three regions, TAG were
faintly labeled, most of the fatty acid being taken up by phospholipids. Caput-derived
cells exhibited the highest rate of lipid labeling. Due to heat exposures,
caput and corpus cells showed a significant increase in the label from [3H]20:4
in diacylglycerols (DAG), concomitant with a decrease in the label in
phosphatidylinositol (PI), phospatidylethanolamine phosphatidylglycerol
(PE-PG), and little change in phosphatidylcholine (PC). Quite the opposite
occurred in cells from cauda: the label in DAG decreased, while that in PI,
PE-PG and PC increased. Our results suggest that epididymal cells are
differentially susceptible to short but periodical exposures to mild
hyperthermia