REGULATION BY MIRNAS OF THE STABILITY OF RAC3 DURING ADIPOGENESIS

MATIAS EZEQUIEL BALDI; MARIA CECILIA LIRA; FRANCISCO DAMIÁN ROSA; MILENI SOARES MACHADO; ALEJANDRA G. PALMA; GABRIELA INES MARINO; MONICA ALEJANDRA COSTAS; MARIA FERNANDA RUBIO

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Congreso; REUNIÓN ANUAL DE SOCIEDADES DE BIOCIENCIA 2020; 2020

Sociedad Argentina de Investigación Clínica

Adipogenesis is regulated by several adipocyte-selective microRNAs(miRNAs) and transcription factors. We have previouslydemonstrated that the expression of the transcriptional coactivatorRAC3 decreases during adipocyte differentiation, favoring the processthrough an increase of autophagy and a decrease in the proliferativerate.In order to determine the mechanism whereby RAC3 expressionlevels are regulated during adipogenesis, we performed publicdatasets analysis and evaluated RAC3 and miRNAs expressionduring adipocyte differentiation (GSE20696 and 20298). After 7days of differentiation, in the murine preadipocyte 3T3-L1 cell line(GSE20696), we observed RAC3 expression was downregulated(43.6% +/- 0.9% respect to control, p1.5-fold change respect to control).We could conclude that the regulation of RAC3 expression duringadipocyte differentiation not only occurs at transcriptional level butalso may involve post-transcriptional regulatory mechanisms th