CROSSTALK BETWEEN ADIPOCYTES AND BREAST CANCER CELLS: MODULATION OF NUCLEAR RECEPTOR COACTIVATOR 3 (RAC3) IN FAT CELLS

LIRA, MARÍA CECILIA; ROSA, FRANCISCO D; SOARES MACHADO, MILENI; PALMA, ALEJANDRA; MARINO, GABRIELA INÉS; COSTAS, MÓNICA A; RUBIO MARÍA FERNANDA

Mar del Plata

Congreso; REUNIÓN ANUAL DE SOCIEDADES DE BIOCIENCIA 2019; 2019

Sociedad Argentina de Investigación Clínica

Adipocytes account for the largest proportion among the cells that comprise breast tissue. Although they are considered to be a critical cell type in the tumor microenvironment of breast cancer, there is still unclear the molecular mechanisms that control their behavior in this context. We have demonstrated that the transcriptional coactivator RAC3 decreases during adipogenesis and its high expression in adipose tissue adjacent to breast tumors correlate with different markers of tumoral progression in patient samples. Therefore, the aim of this work was to further study the role of this molecule in an in vitro model. Conditioned media (CM) were collected from non-tumoral or tumoral breast human cell lines with the following RAC3 mRNA levels: MCF10A: 1.55 ± 0.73, MDA-MB-231: 167.20 ± 6.91, T47D: 571.00 ± 12.59. 3T3-L1 derived-murine adipocytes were stimulated with CM or serum-free medium. RAC3 expression levels measured by qPCR resulted significantly higher in adipocytes stimulated with CM from high RAC3 levels-expressing T47D cells (9.25 ± 1.02) compared to the other conditions: basal (1.00 ± 0.00), MCF10A (2.33 ± 0.96), MDA-MB-231 (2.80 ± 1.19) (p