ANTITUMOR EFFECTS OF ALOYSIA POLYSTACHYA EXTRACT IN COLORECTAL CANCER

MILENI SOARES MACHADO; LAURA PANELO; MARÍA CECILIA LIRA; FRANCISCO DAMIÁN ROSA; ALEJANDRA PALMA; GABRIELA INÉS MARINO; MARÍA FERNANDA RUBIO; ALEJANDRO URTREGER; MÓNICA ALEJANDRA COSTAS

Mar del Plata

Congreso; LXIII Reunión Anual de la Sociedad Argentina de Investigación Clínica; 2018

SAIC

Aloysia Polystachya (AP) is an aromatic native plant of Verbenaceaefamily which is widely distributed in subtropical regions of SouthAmerica and the North of Argentine. Uses in medicine of Aloysiaspecies include diuretic, sedative, antispasmodic activities. We havepreviously demonstrated that AP extracts exert cytotoxic effects inseveral human tumor cell lines, including apoptosis. The aim of thiswork was to investigate if cytotoxic effects of AP extract could beextensive to cancer stem cells (CSC), and also, the possible sensitizationor potentiation of chemotherapeutic drugs, using colorectalcancer model. Therefore, the colorectal cancer cell lines CT26(mouse) and HCT116 (human), were stimulated with AP extractor vehicle, with or without 5-fluorouracyle (5-FURA) and then, thesurviving and CSC properties were determined. The surviving wasmeasured after crystal violet staining at 570nm and CSC phenotypewas determined by measuring the CSC marker CD133 (PCR), colonyformation (clonogenic assay) and the HOESCHT efflux capacity(chemotherapeutic drugs efflux via ABCG2 transporters). We foundthat AP extract (0,0004625 mg/ml of flavonoids) decreased theCD133 expression (98%), the colony formation (p